亚洲精品一线,好吊美这里有精品,αv天堂无码在线,av在线 天天

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>小鼠正常細(xì)胞>CRL-2007 JB6 Cl 30-7b 小鼠表皮細(xì)胞

CRL-2007 JB6 Cl 30-7b 小鼠表皮細(xì)胞

簡要描述:CRL-2007 JB6 Cl 30-7b 小鼠表皮細(xì)胞 ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件,

  • 產(chǎn)品型號:
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2025-06-28
  • 訪  問  量:2942

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2007 JB6 Cl 30-7b 小鼠表皮細(xì)胞

ATCC® Number:CRL-2007™Price:$429.00
Designations:JB6 Cl 30-7b

Depositors:NH Colburn

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:epithelial


Source:Organ: skin
Strain: BALB/c
Tissue: epidermis
Disease: normal


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Tumorigenic:CRL-2007 JB6 Cl 30-7b 小鼠表皮細(xì)胞

Age:newborn

Comments:This is one of several lines that are useful in studies of the molecular events in tumor promotion and for development of promotion assays (see also ATCC CRL-2002 - RT101, ATCC CRL-2010 - JB6 Cl 41-5a and ATCC CRL-2012 - T36274).

The JB6 Cl 30-7b cell line was derived from primary cultures of neonatal BALB/c epidermal cells that had been treated with dimethylsulfoxide (DMSO, 0.01 to 0.1 %).

The line is resistant to promotion of transformation (P-) by phorbol esters.

These cells can be used in comparisons with P+ cells for studying differential responses to tumor promoters.

Never allow the cells to become confluent.

The cells must be carried at low density to avoid spontaneous transformation.



Propagation:ATCC complete growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine, 95%; fetal bovine serum, 5%
Temperature: 37.0°C


Subculturing:Protocol: The cells must be carried at low density to avoid spontaneous transformation.Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 1. Remove and discard culture medium.2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 5. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculation density of 2 x 104 viable cells per 25 sq. cm. flask is recommended or a spilt ratio of 1:6 to 1:8.6. Incubate cultures at 37°C.
Subc*tion Ratio: An inoculation density of 2 X 10 exp4 viable cells per 25 sq. cm. flask is recommended
Medium Renewal: 1 to 2 times per week

Rap the flask smartly against a hard surface to get a single cell suspension.

Add fresh medium, aspirate and dispense into new flasks. Culture at 36C in a humidified atmosphere of 5% CO2 - 95% air.



References:22309: Colburn NH, et al. A cell culture assay for tumor-promoter-dependent progression toward neoplastic phenotype: detection of tumor promoters and promotion inhibitors. Teratog. Carcinog. Mutagen. 1: 87-96, 1980. PubMed: 6119803

22427: Colburn NH, et al. Correlation of anchorage-independent growth with tumorigenicity of chemically transformed mouse epidermal cells. Cancer Res. 38: 624-634, 1978. PubMed: 626967

22908: Colburn NH, et al. Tumour promoter induces anchorage independence irreversibly. Nature 281: 589-591, 1979. PubMed: 492322

22959: Bernstein LR, Colburn NH. AP1/jun function is differentially induced in promotion-sensitive and resistant JB6 cells. Science 244: 566-569, 1989. PubMed: 2541502

23279: Colburn NH, et al. Dissociation of mitogenesis and late-stage promotion of tumor cell phenotype by phorbol esters: mitogen-resistant variants are sensitive to promotion. Proc. Natl. Acad. Sci. USA 78: 6912-6916, 1981. PubMed: 6947266























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
欧美四区七页| 91精品无人成人www| 国产一区极品| 国产极品粉嫩尤物一线天| 1024人妻一区二区三区不卡| 成人免费视频观看99| 亚洲日韩午夜成人中文字幕| 日本三级电影成人网久久| AV网站在线观看 第一 二页| 淫妇淫穴Av| 日本欧美久| 加勒比免费一区二区三区| 欧美黑人中文字幕久久视频| 亚洲毛片AⅤ| 亚洲国产中文字幕99| 国产cd在线porn| 日本一区二区黄色| 女生张开腿国产欧美一区二区| 极品呦呦在线播放| 欧美第一页欧美第一页亚洲| ww亚洲ww免费在线观看| 人妻aV鲁丝| 开心婷婷五月天中文字幕无码| 欧美日韩6区| 日韩欧美久久综合| 极品国产欧美| 亚洲精品中文字一区二区| 亚洲综合一区二区17| 日本国产免费国产| 欧美亚洲日韩成人一区二区| 青艹草av在线播放| 不卡一区二区三区免费| 久国产ww| 亚洲欧美日逼| 狠日狠日国产| 天天色天天日天天日天天射| 国产精品97久久久久久| 日韩美欧一区二区三区| 0930日韩专区在线| 日韩 国产不卡| 人人妻人人麻豆|